CHICAGO (CBS) — On the heels of the controversy of a rant that drew fury at Tracy Morgan of “30 Rock” fame, another comedian has been taken to task for using anti-gay slurs in his act – this time in a Chicago show.

Comedian Jo Koy appeared this past Wednesday evening at the Vic Theatre, 3145 N. Sheffield Ave., as part of the Just for Laughs Comedy Festival.

The Windy City Times reports during his standup routine, he began dancing effeminately and said, “I made this dance extra-gay, since I am not gay.”

Later, the Windy City Times reported, Koy was standing onstage with his zipper open as female fans screamed, and when a man in the audience said, “I saw it,” Koy responded, “What are you looking at, f***ing fa**ot?”

Koy, who is best known for his appearances on Chelsea Handler’s cable talk show, issued an apology via Twitter on Thursday.

“I sincerely apologize for the incredibly foul word I chose to use on stage last night in Chicago. It was a moment that I regret,” he wrote. “I realize that words can be hurtful , which is never my intent. I’m truly saddened if I offended anyone with my poor choice of words, particularly the gay community. Homophobia is not funny.”

The controversy follows another uproar stemming from homophobic remarks made by Morgan earlier this month during a performance in Nashville. The performance reportedly included a remark that if he would stab his son if he were gay.

Morgan also later apologized for the remarks, saying in part, “I am not a hateful person and don’t condone any kind of violence against others,” CBS News reported. Still, he took heat from several other celebrities, including “30 Rock” creator and co-star Tina Fey, who called Morgan’s rant “disturbing to me at a time when homophobic hate crimes continue to be a life-threatening issue for the GLBT Community,” CBS News reported.

Watch & Listen LIVE